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Background  The rate-limiting step that determines the dominant time constant (τ D ) of mammalian rod photoresponse recovery is the deactivation of the active phosphodiesterase (PDE6). Physiologically relevant Ca 2+ -dependent mechanisms that would affect the PDE inactivation have not been identified. However, recently it has been shown that τ D  is modulated by background light in mouse rods.    Methodology/Principal Findings  We used  ex vivo  ERG technique to record pharmacologically isolated photoreceptor responses (fast PIII component). We show a novel static effect of calcium on mouse rod phototransduction: Ca 2+  shortens the dominant time constant (τ D ) of saturated photoresponse recovery, i.e., when extracellular free Ca 2+  is decreased from 1 mM to ∼25 nM, the τ D  is reversibly increased ∼1.5–2-fold.    Conclusions  We conclude that the increase in τ D  during low Ca 2+  treatment is not due to increased [cGMP], increased [Na + ] or decreased [ATP] in rod outer segment (ROS). Also it cannot be due to protein translocation mechanisms. We suggest that a Ca 2+ -dependent mechanism controls the life time of active PDE.

Calcium Sets the Physiological Value of the Dominant Time Constant of Saturated Mouse Rod Photoresponse Recovery