Open AccessAnalysis of Short Tandem Repeats by Parallel DNA Threading
Author(s) -
Paweł Zając,
Christine Öberg,
Afshin Ahmadian
Publication year - 2009
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0007823
Subject(s) - microsatellite , str analysis , tandem repeat , threading (protein sequence) , multiplex , biology , genetics , computational biology , dna sequencing , dna , str multiplex system , capillary electrophoresis , microbiology and biotechnology , genome , gene , protein structure , allele , biochemistry
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.
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