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Background  The  Drosophila  gene  erupted  ( ept ) encodes the fly homolog of human Tumor Susceptibility Gene-101 (TSG101), which functions as part of the conserved ESCRT-1 complex to facilitate the movement of cargoes through the endolysosomal pathway. Loss of  ept  or other genes that encode components of the endocytic machinery (e.g.  synatxin7/avalanche ,  rab5 , and  vps25 ) produces disorganized overgrowth of imaginal disc tissue. Excess cell division is postulated to be a primary cause of these ‘neoplastic’ phenotypes, but the autonomous effect of these mutations on cell cycle control has not been examined.    Principal Findings  Here we show that disc cells lacking  ept  function display an altered cell cycle profile indicative of deregulated progression through the G1-to-S phase transition and express reduced levels of the tumor suppressor ortholog and G1/S inhibitor Rbf1. Genetic reductions of the  Drosophila  aPKC kinase (DaPKC), which has been shown to promote tumor growth in other fly tumor models, prevent both the  ept  neoplastic phenotype and the reduction in Rbf1 levels that otherwise occurs in clones of  ept  mutant cells; this effect is coincident with changes in localization of Notch and Crumbs, two proteins whose sorting is altered in  ept  mutant cells. The effect on Rbf1 can also be blocked by removal of the γ-secretase component  presenilin , suggesting that cleavage of a γ-secretase target influences Rbf1 levels in  ept  mutant cells. Expression of exogenous  rbf1  completely ablates  ept  mutant eye tissues but only mildly affects the development of discs composed of cells with wild type  ept .    Conclusions  Together, these data show that loss of  ept  alters nuclear cell cycle control in developing imaginal discs and identify the  DaPKC ,  presenilin , and  rbf1  genes as modifiers of molecular and cellular phenotypes that result from loss of  ept .

Functional Interactions between the erupted/tsg101 Growth Suppressor Gene and the DaPKC and rbf1 Genes in Drosophila Imaginal Disc Tumors