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Prominin1 (Prom1, also known as CD133 in human) has been widely used as a marker for cancer stem cells (CSCs), which self-renew and are tumorigenic, in malignant tumors including glioblastoma multiforme (GBM). However, there is other evidence showing that Prom1-negative cancer cells also form tumors  in vivo . Thus it remains controversial whether Prom1 is a  bona fide  marker for CSCs. To verify if  Prom1 -expressing cells are essential for tumorigenesis, we established a mouse line, whose  Prom1 -expressing cells can be eliminated conditionally by a Cre-inducible  DTA  gene on the  Prom1  locus together with a tamoxifen-inducible CreER TM , and generated glioma-initiating cells (GICs-LD) by overexpressing both the SV40 Large T antigen and an oncogenic H-Ras L61  in neural stem cells of the mouse line. We show here that the tamoxifen-treated GICs-LD (GICs-DTA) form tumor-spheres in culture and transplantable GBM  in vivo . Thus, our studies demonstrate that  Prom1 -expressing cells are dispensable for gliomagenesis in this mouse model.

Glioblastoma Formation from Cell Population Depleted of Prominin1-Expressing Cells