Open AccessSingle Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
Author(s) -
Roberta Schulte,
Jessica A. Talamas,
Christine Doucet,
Martin W. Hetzer
Publication year - 2008
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0002061
Subject(s) - affinity chromatography , fluorescence , chemistry , bead , protein–protein interaction , protein detection , biophysics , fluorescence microscope , plasma protein binding , chromatography , target protein , microscopy , nanotechnology , biochemistry , biology , materials science , physics , enzyme , composite material , gene , quantum mechanics , optics
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
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