English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Chicken endogenous viruses,  ALVE  (Avian Leukosis Virus subgroup E), are inherited as LTR (long terminal repeat) retrotransposons, which are negatively correlated with disease resistance, and any changes in DNA methylation may contribute to the susceptibility to neoplastic disease. The relationship between  ALVE  methylation status and neoplastic disease in the chicken is undefined. White Leghorn inbred lines 7 2  and 6 3  at the ADOL have been respectively selected for resistance and susceptibility to tumors that are induced by avian viruses. In this study, the DNA methylation patterns of 3∼6 CpG sites of four conserved regions in  ALVE , including one unique region in  ALVE1 , the promoter region in the  TVB  (tumor virus receptor of ALV subgroup B, D and E) locus, were analyzed in the two lines using pyrosequencing methods in four tissues,  i.e ., liver, spleen, blood and hypothalamus. A significant CpG hypermethylation level was seen in line 7 2  in all four tissues,  e.g ., 91.86±1.63% for  ALVE  region2 in blood, whereas the same region was hemimethylated (46.16±2.56%) in line 6 3 . CpG methylation contents of the  ALVE  regions were significantly lower in line 6 3  than in line 7 2  in all tissues ( P <0.01) except the  ALVE  region 3/4 in liver. RNA expressions of  ALVE  regions 2 and 3 (PPT-U3) were significantly higher in line 6 3  than in line 7 2  ( P <0.01). The methylation levels of six recombinant congenic strains (RCSs) closely resembled to the background line 6 3  in  ALVE -region 2, which imply the methylation pattern of  ALVE -region 2 may be a biomarker in resistant disease breeding. The methylation level of the promoter region in the  TVB  was significantly different in blood ( P <0.05) and hypothalamus ( P <0.0001), respectively. Our data disclosed a hypermethylation pattern of  ALVE  that may be relevant for resistance against ALV induced tumors in chickens.

Quantitative Evaluation of DNA Methylation Patterns for ALVE and TVB Genes in a Neoplastic Disease Susceptible and Resistant Chicken Model