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A  Trypanosoma cruzi  Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a convenience series of clinical samples (venous blood treated with EDTA or different stabilizer agents, heel-prick blood in filter paper or cerebrospinal fluid samples (CSF)) from 30 infants born to seropositive mothers (13 with congenital CD and 17 noninfected), four recipients of organs from CD donors, six orally–infected cases after consumption of contaminated guava juice and six CD patients coinfected with HIV at risk of CD reactivation (N = 46 patients, 46 blood samples and 1 CSF sample) were tested by  T .  cruzi  Loopamp kit ( Tc  LAMP) and standardized quantitative real-time PCR (qPCR).  T .  cruzi  Loopamp accuracy was estimated using the case definition in the different groups as a reference. Cohen’s kappa coefficient (κ) was applied to measure the agreement between  Tc  LAMP (index test) and qPCR (reference test). Sensitivity and specificity of  T .  cruzi  Loopamp kit in blood samples from the pooled clinical groups was 93% (95% CI: 77–99) and 100% (95% CI: 80–100) respectively. The agreement between  Tc  LAMP and qPCR was almost perfect (κ = 0.92, 95% CI: 0.62–1.00). The  T .  cruzi  Loopamp kit was sensitive and specific for detection of  T .  cruzi  infection. It was carried out from DNA extracted from peripheral blood samples (via frozen EDTA blood, guanidine hydrochloride-EDTA blood, DNAgard blood and dried blood spots), as well as in CSF specimens infected with TcI or TcII/V/VI parasite populations. The  T .  cruzi  Loopamp kit appears potentially useful for rapid detection of  T .  cruzi  infection in congenital, acute and CD reactivation due to HIV infection.

plos neglected tropical diseases

Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation