English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Recent epidemics of Zika virus (ZIKV) in the Pacific and the Americas have highlighted its potential as an emerging pathogen of global importance. Both  Aedes (Ae . ) aegypti  and  Ae .  albopictus  are known to transmit ZIKV but variable vector competence has been observed between mosquito populations from different geographical regions and different virus strains. Since Australia remains at risk of ZIKV introduction, we evaluated the vector competence of local  Ae .  aegypti  and  Ae .  albopictus  for a Brazilian epidemic ZIKV strain. In addition, we evaluated the impact of daily temperature fluctuations around a mean of 28°C on ZIKV transmission and extrinsic incubation period.    Methodology/Principal findings  Mosquitoes were orally challenged with a Brazilian ZIKV strain (8.8 log CCID 50 /ml) and maintained at either 28°C constant or fluctuating temperature conditions. At 3, 7 and 14 days post-infection (dpi), ZIKV RNA copies were quantified in mosquito bodies, as well as wings and legs, using qRT-PCR, while virus antigen in saliva (a proxy for transmission) was detected using a cell culture ELISA. Despite high body and disseminated infection rates in both vectors, the transmission rates of ZIKV in saliva of  Ae .  aegypti  (50–60%) were significantly higher than in  Ae .  albopictus  (10%) at 14 dpi. Both species supported a high viral load in bodies, with no significant differences between constant and fluctuating temperature conditions. However, a significant difference in viral load in wings and legs between species was observed, with higher titres in  Ae .  aegypti  maintained at constant temperature conditions. For ZIKV transmission to occur in  Ae .  aegypti , a disseminated virus load threshold of 7.59 log 10  copies had to be reached.    Conclusions/Significance  Australian  Ae .  aegypti  are better able to transmit a Brazilian ZIKV strain than  Ae .  albopictus . The results are in agreement with the global consensus that  Ae .  aegypti  is the major vector of ZIKV.

Vector competence of Australian Aedes aegypti and Aedes albopictus for an epidemic strain of Zika virus