English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background/Methodology  Triatomine bugs are the vectors of  Trypanosoma cruzi , the agent of Chagas disease. Vector control has for decades relied upon insecticide spraying, but insecticide resistance has recently emerged in several triatomine populations. One alternative strategy to reduce  T .  cruzi  transmission is paratransgenesis, whereby symbiotic bacteria are genetically engineered to produce  T .  cruzi -killing proteins in the vector’s gut. This approach requires in-depth knowledge of the vectors’ natural gut microbiota. Here, we use metagenomics (16S rRNA 454 pyrosequencing) to describe the gut microbiota of field-caught  Triatoma sordida –likely the most common peridomestic triatomine in Brazil. For large nymphs (4 th  and 5 th  stage) and adults, we also studied separately the three main digestive-tract segments–anterior midgut, posterior midgut, and hindgut.    Principal findings  Bacteria of four phyla (12 genera) were present in both nymphs (all five stages) and adults, thus defining  T .  sordida ’s ‘bacterial core’: Actinobacteria ( Brevibacterium ,  Corynebacterium ,  Dietzia ,  Gordonia ,  Nitriliruptor ,  Nocardia ,  Nocardiopsis ,  Rhodococcus , and  Williamsia ), Proteobacteria ( Pseudomonas  and  Sphingobium ), and Firmicutes ( Staphylococcus ). We found some clear differences in bacterial composition and relative abundance among development stages; overall, Firmicutes and Proteobacteria increased, but Actinobacteria decreased, through development. Finally, the bacterial microbiotas of the bugs’ anterior midgut, posterior midgut, and hindgut were sharply distinct.    Conclusions/Significance  Our results identify the ‘bacterial core set’ of  T .  sordida  and reveal important gut microbiota differences among development stages–particularly between 1 st –3 rd  stage nymphs and adults. Further, we show that, within any given development stage, the vectors’ gut cannot be regarded as a single homogeneous environment. Cultivable, non-pathogenic ‘core’ bacterial species may now be tested as candidates for paratransgenic control of  T .  cruzi  transmission by  T .  sordida .

plos neglected tropical diseases

Field-collected Triatoma sordida from central Brazil display high microbiota diversity that varies with regard to developmental stage and intestinal segmentation