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Emergomyces africanus  is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5  Es .  africanus  propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected  Es .  africanus  in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne  Es .  africanus  propagules occurs more commonly in endemic areas than previously appreciated.

Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa