English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The human genome encodes thousands of long noncoding RNA (lncRNA) genes; the function of majority of them is poorly understood. Aberrant expression of a significant number of lncRNAs is observed in various diseases, including cancer. To gain insights into the role of lncRNAs in breast cancer progression, we performed genome-wide transcriptome analyses in an isogenic, triple negative breast cancer (TNBC/basal-like) progression cell lines using a 3D cell culture model. We identified significantly altered expression of 1853 lncRNAs, including ~500 natural antisense transcript (NATs) lncRNAs. A significant number of breast cancer-deregulated NATs displayed co-regulated expression with oncogenic and tumor suppressor protein-coding genes in  cis . Further studies on one such NAT,  PDCD4-AS1  lncRNA reveal that it positively regulates the expression and activity of the tumor suppressor  PDCD4  in mammary epithelial cells. Both  PDCD4-AS1  and  PDCD4  show reduced expression in TNBC cell lines and in patients, and depletion of  PDCD4-AS1  compromised the cellular levels and activity of PDCD4. Further, tumorigenic properties of  PDCD4-AS1 -depleted TNBC cells were rescued by exogenous expression of  PDCD4 , implying that  PDCD4-AS1  acts upstream of  PDCD4 . Mechanistically,  PDCD4-AS1  stabilizes  PDCD4  RNA by forming RNA duplex and controls the interaction between  PDCD4  RNA and RNA decay promoting factors such as HuR. Our studies demonstrate crucial roles played by NAT lncRNAs in regulating post-transcriptional gene expression of key oncogenic or tumor suppressor genes, thereby contributing to TNBC progression.

A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability