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The insertion Sequence IS 6110 , only present in the pathogens of the  Mycobacterium tuberculosis  Complex (MTBC), has been the gold-standard epidemiological marker for TB for more than 25 years, but biological implications of IS 6110  transposition during MTBC adaptation to humans remain elusive. By studying 2,236 clinical isolates typed by IS 6110 -RFLP and covering the MTBC, we remarked a lineage-specific content of IS 6110  being higher in modern globally distributed strains. Once observed the IS 6110  distribution in the MTBC, we selected representative isolates and found a correlation between the normalized expression of IS 6110  and its abundance in MTBC chromosomes. We also studied the molecular regulation of IS 6110  transposition and we found a synergistic action of two post-transcriptional mechanisms: a -1 ribosomal frameshift and a RNA pseudoknot which interferes translation. The construction of a transcriptionally active transposase resulted in 20-fold increase of the transposition frequency. Finally, we examined transposition in  M .  bovis  and  M .  tuberculosis  during laboratory starvation and in a mouse infection model of TB. Our results shown a higher transposition in  M .  tuberculosis , that preferably happens during TB infection in mice and after one year of laboratory culture, suggesting that IS 6110  transposition is dynamically adapted to the host and to adverse growth conditions.

New insights into the transposition mechanisms of IS6110 and its dynamic distribution between Mycobacterium tuberculosis Complex lineages