English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The cell envelope of mycobacteria is a highly unique and complex structure that is functionally equivalent to that of Gram-negative bacteria to protect the bacterial cell. Defects in the integrity or assembly of this cell envelope must be sensed to allow the induction of stress response systems. The promoter that is specifically and most strongly induced upon exposure to ethambutol and isoniazid, first line drugs that affect cell envelope biogenesis, is the  iniBAC  promoter. In this study, we set out to identify the regulator of the  iniBAC  operon in  Mycobacterium marinum  using an unbiased transposon mutagenesis screen in a constitutively  iniBAC -expressing mutant background. We obtained multiple mutants in the  mce1  locus as well as mutants in an uncharacterized putative transcriptional regulator ( MMAR_0612 ). This latter gene was shown to function as the  iniBAC  regulator, as overexpression resulted in constitutive  iniBAC  induction, whereas a knockout mutant was unable to respond to the presence of ethambutol and isoniazid. Experiments with the  M .  tuberculosis  homologue ( Rv0339c ) showed identical results. RNAseq experiments showed that this regulatory gene was exclusively involved in the regulation of the  iniBAC  operon. We therefore propose to name this dedicated regulator   ini   BAC   R egulator (IniR). IniR belongs to the family of signal transduction ATPases with numerous domains, including a putative sugar-binding domain. Upon testing different sugars, we identified trehalose as an activator and metabolic cue for  iniBAC  activation, which could also explain the effect of the  mce1  mutations. In conclusion, cell envelope stress in mycobacteria is regulated by IniR in a cascade that includes trehalose.

Cell envelope stress in mycobacteria is regulated by the novel signal transduction ATPase IniR in response to trehalose