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Several nucleoporins in the nuclear pore complex (NPC) have been reported to be involved in abiotic stress responses in plants. However, the molecular mechanism of how NPC regulates abiotic stress responses, especially the expression of stress responsive genes remains poorly understood. From a forward genetics screen using an abiotic stress-responsive luciferase reporter ( RD29A-LUC ) in the  sickle-1  ( sic-1 ) mutant background, we identified a suppressor caused by a mutation in  NUCLEOPORIN 85  ( NUP85 ), which exhibited reduced expression of  RD29A-LUC  in response to ABA and salt stress. Consistently, the ABA and salinity induced expression of several stress responsive genes such as  RD29A ,  COR15A  and  COR47  was significantly compromised in  nup85  mutants and other nucleoporin mutants such as  nup160  and  hos1 . Subsequently, Immunoprecipitation and mass spectrometry analysis revealed that NUP85 is potentially associated with HOS1 and other nucleoporins within the nup107-160 complex, along with several mediator subunits. We further showed that there is a direct physical interaction between MED18 and NUP85. Similar to  NUP85  mutations,  MED18  mutation was also found to attenuate expression of stress responsive genes. Taken together, we not only revealed the involvement of  NUP85  and other nucleoporins in regulating ABA and salt stress responses, but also uncovered a potential relation between NPC and mediator complex in modulating the gene expression in plants.

An Arabidopsis Nucleoporin NUP85 modulates plant responses to ABA and salt stress