English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm,  Bombyx mori , has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the  B .  mori  Fem piRNA as the primary female determining factor and  BmMasc  as its downstream target, while the genetic scenario for male sex determination was still unclear. In the current study, we exploite the transgenic CRISPR/Cas9 system to generate a comprehensive set of knockout mutations in genes  BmSxl ,  Bmtra2 ,  BmImp ,  BmImp   M  ,  BmPSI  and  BmMasc , to investigate their roles in silkworm sex determination. Absence of  Bmtra2  results in the complete depletion of  Bmdsx  transcripts, which is the conserved downstream factor in the sex determination pathway, and induces embryonic lethality. Loss of  BmImp  or  BmImp   M   function does not affect the sexual differentiation. Mutations in  BmPSI  and  BmMasc  genes affect the splicing of  Bmdsx  and the female reproductive apparatus appeared in the male external genital. Intriguingly, we identify that  BmPSI  regulates expression of  BmMasc ,  BmImp   M   and  Bmdsx , supporting the conclusion that it acts as a key auxiliary factor in silkworm male sex determination.

Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination