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Catenation links between sister chromatids are formed progressively during DNA replication and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in  E .  coli  using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the  E .  coli  chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chromatin and gene expression context influence site selection. Moreover strong DNA-binding and catalytic activities are found at the chromosome dimer resolution site,  dif , located opposite the origin of replication. We reveal a physical and functional interaction between Topo IV and the XerCD recombinases acting at the  dif  site. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/ dif  and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.

Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome