English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The exclusive localization of the histone H3 variant CENP-A to centromeres is essential for accurate chromosome segregation. Ubiquitin-mediated proteolysis helps to ensure that CENP-A does not mislocalize to euchromatin, which can lead to genomic instability. Consistent with this, overexpression of the budding yeast CENP-A Cse4  is lethal in cells lacking Psh1, the E3 ubiquitin ligase that targets CENP-A Cse4  for degradation. To identify additional mechanisms that prevent CENP-A Cse4  misincorporation and lethality, we analyzed the genome-wide mislocalization pattern of overexpressed CENP-A Cse4  in the presence and absence of Psh1 by chromatin immunoprecipitation followed by high throughput sequencing. We found that ectopic CENP-A Cse4  is enriched at promoters that contain histone H2A.Z Htz1  nucleosomes, but that H2A.Z Htz1  is not required for CENP-A Cse4  mislocalization. Instead, the INO80 complex, which removes H2A.Z Htz1  from nucleosomes, promotes the ectopic deposition of CENP-A Cse4 . Transcriptional profiling revealed gene expression changes in the  psh1Δ  cells overexpressing CENP-A Cse4 . The down-regulated genes are enriched for CENP-A Cse4  mislocalization to promoters, while the up-regulated genes correlate with those that are also transcriptionally up-regulated in an  htz1Δ  strain. Together, these data show that regulating centromeric nucleosome localization is not only critical for maintaining centromere function, but also for ensuring accurate promoter function and transcriptional regulation.

Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects