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The genome of the fission yeast  Schizosaccharomyces pombe  encodes 17 kinases that are essential for cell growth. These include the cell-cycle regulator Cdc2, as well as several kinases that coordinate cell growth, polarity, and morphogenesis during the cell cycle. In this study, we further characterized another of these essential kinases, Prp4, and showed that the splicing of many introns is dependent on Prp4 kinase activity. For detailed characterization, we chose the genes  res1  and  ppk8 , each of which contains one intron of typical size and position. Splicing of the  res1  intron was dependent on Prp4 kinase activity, whereas splicing of the  ppk8  intron was not. Extensive mutational analyses of the 5’ splice site of both genes revealed that proper transient interaction with the 5’ end of snRNA U1 governs the dependence of splicing on Prp4 kinase activity. Proper transient interaction between the branch sequence and snRNA U2 was also important. Therefore, the Prp4 kinase is required for recognition and efficient splicing of introns displaying weak exon1/5’ splice sites and weak branch sequences.

Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation