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The fission yeast  Schizosaccharomyces pombe  Rec12 protein, the homolog of Spo11 in other organisms, initiates meiotic recombination by creating DNA double-strand breaks (DSBs) and becoming covalently linked to the DNA ends of the break. This protein–DNA linkage has previously been detected only in mutants such as  rad50S  in which break repair is impeded and DSBs accumulate. In the budding yeast  Saccharomyces cerevisiae , the DSB distribution in a  rad50S  mutant is markedly different from that in wild-type ( RAD50 ) meiosis, and it was suggested that this might also be true for other organisms. Here, we show that we can detect Rec12-DNA linkages in  Sc. pombe rad50 +   cells, which are proficient for DSB repair. In contrast to the results from  Sa. cerevisiae , genome-wide microarray analysis of Rec12-DNA reveals indistinguishable meiotic DSB distributions in  rad50 +   and  rad50S  strains of  Sc. pombe . These results confirm our earlier findings describing the occurrence of widely spaced DSBs primarily in large intergenic regions of DNA and demonstrate the relevance and usefulness of fission yeast studies employing  rad50S . We propose that the differential behavior of  rad50S  strains reflects a major difference in DSB regulation between the two species—specifically, the requirement for the Rad50-containing complex for DSB formation in budding yeast but not in fission yeast. Use of  rad50S  and related mutations may be a useful method for DSB analysis in other species.

Indistinguishable Landscapes of Meiotic DNA Breaks in rad50+ and rad50S Strains of Fission Yeast Revealed by a Novel rad50+ Recombination Intermediate