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Experiments of pain with human subjects are difficult, subjective, andethically constrained. Since the molecular mechanisms of pain transduction arereasonably conserved among different species, these problems are partiallysolved by the use of animal models. However, animals cannot easily communicateto us their own pain levels. Thus progress depends crucially on our ability toquantitatively and objectively infer the perceived level of noxious stimulifrom the behavior of animals. Here we develop a quantitative model to infer theperceived level of thermal nociception from the stereotyped nociceptiveresponse of individual nematodes Caenorhabditis elegans stimulated by an IRlaser. The model provides a method for quantification of analgesic effects ofchemical stimuli or genetic mutations in C. elegans. We test the nociception ofibuprofen-treated worms and a TRPV (transient receptor potential) mutant, andwe show that the perception of thermal nociception for the ibuprofen treatedworms is lower than the wild-type. At the same time, our model shows that themutant changes the worm's behavior beyond affecting nociception. Finally, wedetermine the stimulus level that best distinguishes the analgesic effects andthe minimum number of worms that allow for a statistically significantidentification of these effects.

Stereotypical escape behavior in Caenorhabditis elegans allows quantification of nociceptive stimuli levels