Open AccessIntroduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level
Author(s) -
Seung-Won Lee,
Seung-Won Lee,
Song Eun Lee,
Song Eun Lee,
Seong Hyuk Ko,
Eun Kyoung Hong,
Kwang Il Nam,
Keiichiro Nakamura,
Shuhei Imayama,
Yeong-Joon Park,
Kyu Youn Ahn,
Choon Sang Bae,
Baik Yoon Kim,
Sung-Sik Park
Publication year - 2005
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1369/jhc.3b6194.2005
Subject(s) - immunocytochemistry , electron microscope , staining , golgi apparatus , chemistry , microbiology and biotechnology , cell , biology , physics , optics , biochemistry , genetics , endocrinology
The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.
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