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We discuss recently emerging applications of the state-of-art deep learningmethods on optical microscopy and microscopic image reconstruction, whichenable new transformations among different modes and modalities of microscopicimaging, driven entirely by image data. We believe that deep learning willfundamentally change both the hardware and image reconstruction methods used inoptical microscopy in a holistic manner.

Toward a Thinking Microscope: Deep Learning in Optical Microscopy and Image Reconstruction