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We propose a novel OCT-based method for visualizing microvasculature in 3Dusing reference-free processing of individual complex-valued B-scans withhighly overlapped A-scans. In the lateral direction of such a B-scan, theamplitude and phase of speckles corresponding to vessel regions exhibit fastervariability, and thus can be detected without comparison with other B-scansrecorded in the same plane. This method combines elements of several existingOCT angiographic approaches, and exhibits: (i) enhanced robustness with respectto bulk tissue motion with frequencies up to tens of Hz; (ii) resolution ofmicrocirculation images equal to that of structural images and (iii)possibility of quantifying the vessels in terms of their decorrelation rates.

Hybrid M-mode-like OCT imaging of 3D microvasculature in vivo using reference-free processing of complex-valued B-scans