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We report an angle-tilted, wavelength-multiplexed ptychographic modulationapproach for multispectral lensless on-chip microscopy. In this approach, weilluminate the specimen with lights at 5 wavelengths simultaneously. A prism isadded at the illumination path for spectral dispersion. Lightwaves at differentwavelengths, thus, hit the specimen at slightly different incident angles,breaking the ambiguities in mixed state ptychographic reconstruction. At thedetection path, we place a thin diffuser in-between the specimen and themonochromatic image sensor for encoding the spectral information into 2Dintensity measurements. By scanning the sample to different x-y positions, weacquire a sequence of monochromatic images for reconstructing the 5 complexobject profiles at the 5 wavelengths. An up-sampling procedure is integratedinto the recovery process to bypass the resolution limit imposed by the imagerpixel size. We demonstrate a half-pitch resolution of 0.55 microns using animage sensor with 1.85-micron pixel size. We also demonstrate quantitative andhigh-quality multispectral reconstructions of stained tissue sections fordigital pathology applications.

Super-resolved multispectral lensless microscopy via angle-tilted, wavelength-multiplexed ptychographic modulation