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We have implemented a multi-functional optofluidic sensor that can monitorchanges in the refractive index and pressure of biofluid simultaneously and candetect free-solution molecular interaction in-situ. In this paper, wedemonstrate two major improvements of this sensor proven by both simulation andexperiments. One improvement is the broader measurement range of refractiveindex by making the diffraction grating with high-index polymer. The otherimprovement is the separation of refractive index sensing from opacity sensingby using the relative power ratio of diffraction orders. This simple, compactand low-cost multi-functional optofluidic sensor has the potential to be usedfor in-situ biofluid monitoring.

Liquid refractive index sensing independent of opacity using an optofluidic sensor based on diffraction