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We present the first evidence of nitrogen vacancy (NV) photoluminescence froma nanodiamond suspended in a free-space optical dipole trap at atmosphericpressure. The photoluminescence rates are shown to decrease with increasingtrap laser power, but are inconsistent with a thermal quenching process. For acontinuous-wave trap, the neutral charge state (NV$^0$) appears to besuppressed. Chopping the trap laser yields higher total count rates and resultsin a mixture of both NV$^0$ and the negative charge state (NV$^-$).

Observation of nitrogen vacancy photoluminescence from an optically levitated nanodiamond