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We introduce a full field fluorescence imaging technique with axialconfinement of about 100 nm at the sample/substrate interface. Contrary tostandard surface imaging techniques, this confinement is obtained throughemission filtering. This technique is based on supercritical emissionselectivity. It can be implemented on any epifluorescence microscope with acommercial high numerical aperture objective and offers a real time surfaceimaging capability. This technique is of particular interest for live cellmembrane and adhesion studies. Using HEK cells, we show that one can observesimultaneously the surface and in-depth cell phenomena.

Full Field Supercritical Angle Fluorescence Microscopy for live cell imaging