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In this study, near-IR tomography was implemented in the wavelength range from 661 to 948 nm to characterize breast tumors in vivo. Frequency-domain measurements provide amplitude and phase transmitted at wavelengths below 850 nm, where photomultiplier tube detection is efficient. Continuous-wave detection at additional longer wavelengths (903, 912, and 948 nm) was collected using a CCD-based spectrometer. Phantom validation experiments showed improved accuracy in hemoglobin and water concentrations using this technique. Three women with malignant breast tumors were studied. The addition of cw data at longer wavelengths increased the recovered contrast of water in the tumor region relative to surrounding tissue and allowed quantification of lipid.

Near-infrared tomography of breast cancer hemoglobin, water, lipid, and scattering using combined frequency domain and cw measurement