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Multi-spectral quantitative phase imaging (QPI) is an emerging imagingmodality for wavelength dependent studies of several biological and industrialspecimens. Simultaneous multi-spectral QPI is generally performed with colorCCD cameras. However, color CCD cameras are suffered from the color crosstalkissue, which needed to be explored. Here, we present a new approach foraccurately measuring the color crosstalk of 2D area detectors, without needingprior information about camera specifications. Color crosstalk of two differentcameras commonly used in QPI, single chip CCD (1-CCD) and three chip CCD(3-CCD), is systematically studied and compared using compact interferencemicroscopy. The influence of color crosstalk on the fringe width and thevisibility of the monochromatic constituents corresponding to three colorchannels of white light interferogram are studied both through simulations andexperiments. It is observed that presence of color crosstalk changes the fringewidth and visibility over the imaging field of view. This leads to an unwantednon-uniform background error in the multi-spectral phase imaging of thespecimens. It is demonstrated that the color crosstalk of the detector is thekey limiting factor for phase measurement accuracy of simultaneousmulti-spectral QPI systems.

Characterization of color cross-talk of CCD detectors and its influence in multispectral quantitative phase imaging