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Fluorescence microscopy is widely used in biological imaging, howeverscattering from tissues strongly limits its applicability to a shallow depth.In this work we adapt a methodology inspired from stellar speckleinterferometry, and exploit the optical memory effect to enable fluorescencemicroscopy through a turbid layer. We demonstrate efficient reconstruction ofmicrometer-size fluorescent objects behind a scattering medium inepi-microscopy, and study the specificities of this imaging modality(magnification, field of view, resolution) as compared to traditionalmicroscopy. Using a modified phase retrieval algorithm to reconstructfluorescent objects from speckle images, we demonstrate robust reconstructionseven in relatively low signal to noise conditions. This modality isparticularly appropriate for imaging in biological media, which are known toexhibit relatively large optical memory ranges compatible with tens ofmicrometers size field of views, and large spectral bandwidths compatible withemission fluorescence spectra of tens of nanometers widths.

Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations