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We propose a strategy for quantitative photoacoustic mapping of chromophoreconcentrations that can be performed purely experimentally. We exploit thepossibility of acousto-optic modulation using focused ultrasound, and theprinciple that photons follow trajectories through a turbid medium in twodirections with equal probability. A theory is presented that expresses thelocal absorption coefficient inside a medium in terms of noninvasively measuredquantities and experimental parameters. Proof of the validity of the theory isgiven with Monte Carlo simulations.

Towards quantitative tissue absorption imaging by combining photoacoustics and acousto-optics