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This paper describes an imaging microscopic technique based on heterodynedigital holography where subwavelength-sized gold colloids can be imaged incell environment. Surface cellular receptors of 3T3 mouse fibroblasts arelabeled with 40 nm gold nanoparticles, and the biological specimen is imaged ina total internal reflection configuration with holographic microscopy. Due to ahigher scattering efficiency of the gold nanoparticles versus that of cellularstructures, accurate localization of a gold marker is obtained within a 3Dmapping of the entire sample's scattered field, with a lateral precision of 5nm and 100 nm in the x,y and in the z directions respectively, demonstratingthe ability of holographic microscopy to locate nanoparticles in living cellsenvironments.

Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy