English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

We present a technically simple implementation of quantitative phase imagingin confocal microscopy based on synthetic optical holography withsinusoidal-phase reference waves. Using a Mirau interference objective andlow-amplitude vertical sample vibration with a piezo-controlled stage, werecord synthetic holograms on commercial confocal microscopes (Nikon, model:A1R; Zeiss: model: LSM-880), from which quantitative phase images arereconstructed. We demonstrate our technique by stain-free imaging of cervical(HeLa) and ovarian (ES-2) cancer cells and stem cell (mHAT9a) samples. Ourtechnique has the potential to extend fluorescence imaging applications inconfocal microscopy by providing label-free cell finding, monitoring cellmorphology, as well as non-perturbing long-time observation of live cells basedon quantitative phase contrast.

Accessible quantitative phase imaging in confocal microscopy with sinusoidal-phase synthetic optical holography