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Our research team has isolated nivalenol, fusarenon-X, diacetylnivalenol, neosolaniol, 4-deoxynivalenol and 4-deoxy-3-acetylnivalenol from strains of Fusarium collected from rice, barley, cereals and pasture. The 12,13-epoxytrichothecenes, after trimethylsilylation of the trichothecene compounds, can be chemically detected to the order of 1 μg ml-1 using gas chromatography and mass spectroscopy. A biological assay method using HeLa cells, including morphological mode of cell damage and also biochemical disturbances in not only protein synthesis but also DNA synthesis using radioactive precursors on the same cell culture system, is also presented.

Chemical and biological detection of 12,13-epoxytrichothecenes isolated from Fusarium species