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Laboratory diagnosis of dengue infection plays an important role for case nlanagenrent and disease control. Unfortunately, many of the available laboratory tests are not appropriate in tet'ms of rapidity and .simplicity. H ere, we report a new format of semiquantitative dot immunoassay for detection of IgM-anti dengue antibodies in human sera entltloyittg biotinylated-dengue antigens which is simple in term of methodoLogy and rapid in ternt of the lest result. Tlrc results indicated that tlrc new test is sensitive for diagnosis of secondary dengue infection. The test result can be obtained withinfour and a halfhours.

Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera