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Limitation of fuels reserves and contribution of fossil fuels to the greenhouse effect leads to develop a new, clean and sustainable energy. Among the various options, biohydrogen appears as a promising alternative energy source. The fermentative hydrogen production process holds a great promise for commercial processes. Hydrogen production by fermentative bacteria is a very complex and greatly influenced by pH. This paper presents biohydrogen production by bacterial strain Clostridium sp. Tr2. Operational pH strongly affected its hyrogen production. Its gas production rate as well as obtained gas product were roughly increase twice under controlled pH at 6 than non-controlled condition. Dark fermentation for hydrogen production of strain Tr2 was performed under bottle as well as automatic fermenter scale under optimal nutritional and environmental conditions at 30C, initial pH at 6.5, then pH was controlled at 6 for bioreactor scale (BioFlo 110). Bioreactor scale was much better for hydrogen production of strain Tr2. Clostridium sp. Tr2 produced 0.74 L hydro (L medium) occupying 72.6 % of total gas under bottle scale while it produced 2.94 L hydro (L medium) occupying 95.82 % of total gas under fermenter scale. Its maximum obtained hydrogen yield of Clostridium sp. Tr2 under bioreactor scale Bioflo 110 in optimal medium with controlled pH 6 was 2.31 mol hydro (mol glucose).

Biohydrogen production by fermentive bacterium Clostridium sp. Tr2 using batch fermenter system controlled pH under dark fermentation