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Formed by a kind of bacteria called Gluconacetobacter, bacterial cellulose (biocellulose, BC) membrane, compared to cellulose from plants, has superior properties for the strength, toughness, durability and elasticity. The subjects of this study are bacteria being able to produce Bacterial cellulose in Spirulina medium. The study aims to investigate the influences of some ecological factors on the Bacterial cellulose membrane forming process in Spirulina medium, and then find out appropriate nutritional media and conditions for the fermentation in Bacterial cellulose forming process. The study has some major findings: (1) Select two strains of bacteria: Gluconacetobacter xylinus T6 and Gluconacetobacter xylinus T9, which prove to be capable of producing cellulose membrane to be used in making nourishing face masks for its thinness, smoothness, toughness and uniformity; (2) Find out the appropriate medium for the formation of Bacterial cellulose membrane, including (NH4)2SO4: 0,5 (g), KH2PO4: 1 (g), glucose: 10 (g), algae powder: 20 (g), and distilled water: 1000 (ml). Successful fermentation for membrane production could be done in appropriate pH of 5 and appropriate temperature of 32C. The ratio of surface area per volume of fermentation (S/V) is 0.8, and the membrane can be collected after 5 days.

Influences of some ecological factors on bacterial cellulose (BC) membrane forming process in Spirulina medium