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This study was conducted to simultaneous analysis methods for water soluble vitamins B group (vitamin B1, vitamin B2, nicotinic acid, nicotinamide, vitamin B6) which is used as health functional foods etc. Ana- lytical methods of water-soluble vitamins B group by HPLC were established through instrumental analytical condi- tions, and the examination of data such as domestic and foreign reliable methods, and papers of journal. HPLC method analyzing water soluble vitamins B group was established using Capcell Pak C18 UG 120 column in 270 nm through test of columns. The validation has been performed on the method to determine linearity, accuracy, limits of quantifi- cation (LOQ) and repeatability for water soluble vitamins B group. An excellent linearity (r 2 = 0.999) was observed for vitamin B1, vitamin B2, nicotinic acid, nicotinamide, vitamin B6 in the concentration range (0.1~2 µg/mL). Observed recovery of vitamin B1 was found to be between 100 and 103%, vitamin B2 was found to be between 104 and 112%, nicotinic acid was found to be between 82 and 85%, nicotinamide was found to be between 121 and 124% and vitamin B6 was found to be between 95 and 104%. LOQ of vitamin B1 was found to be 0.04 µg/mL, vitamin B2 was found to be 0.05 µg/mL, nicotinic acid was found to be 0.15 µg/mL, nicotinamide was found to be 0.08 µg/mL and vitamin B6 was found to be 0.63 µg/mL. Repeatability precision for vitamin B1 was found to be 0.4%, vitamin B2 was found to be 0.4%, nicotinic acid was found to be 0.5%, nicotinamide was found to be 0.7% and vitamin B6 was found to be 0.4% relative standard deviation (RSD). Also, verify the accuracy of the simultaneous analysis methods, we monitored the labeled contents of the health functional foods and children's preferred foods.

Simultaneous Determination of Water Soluble Vitamins B Group in Health Functional Foods etc. by HPLC