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Stevioside, a natural sweeteners presently used in various kinds of food and food products in Korea, was evaluated for its toxicity potential in the 14 day feeding study using B6C3F1 mice. Stevioside was added to the diet at different concentrations of 0.31, 0.62, 1.25, 2.5 and 5%, and was administered for 14 consecutive days. An increase of liver organ weight in male mice was observed. No diet-related differences were noted in clinical signs, food consumption, and gross and histopatholgical evaluation. Based on these results, we concluded that the concen- tration of 5% in the diet was a suitable maximum tolerable dose of stevioside for a 90 day study in mice.

Increase of Liver Organ Weight in B6C3F1 Mice Fed with High dose Stevioside for 14 Days