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Objective: To compare the efficacy of Chicago sky blue (CSB) stain with the routine potassium hydroxide (KOH) wet mount in the diagnosis of dermatophytosis and pityriasis versicolor.Methods: Duplicate skin scrapings from patients with a clinical diagnosis of dermatophyte infection and pityriasis versicolor were examined with the KOH wet mount and the CSB stain.Results: Thirty-six patients had dermatophyte infections, and 13 patients had pityriasis versicolor. After 30 minutes, 13 (36%) of 36 dermatophyte slides and 2 (15%) of 13 pityriasis versicolor slides became positive with 20% KOH. The corresponding data for CSB stain were 23 (64%) of 36 dermatophyte slides and 10 (77%) of 13 pityriasis versicolor slides.Conclusion: Chicago sky blue stain is superior to the KOH wet mount for the diagnosis of dermatophyte infection and pityriasis versicolor and is equally inexpensive. We recommend reading negative dermatophyte slides again on Day 2, but a 30-minute reading is adequate for pityriasis versicolor.* CSB : Chicago sky blue KOH : potassium hydroxide PAS : periodic acid-Schiff PCR : polymerase chain reaction

New Contrast Stain for the Rapid Diagnosis of Dermatophytosis and Pityriasis Versicolor