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High-performance liquid chromatography (HPLC) was used to identify and quantify fifteen amino acids and phenolic active compounds in the tubers of twelve potato (Solanum tuberosum L.) varieties: very early – ‘VB Venta’, ‘Fresco’, ‘Acapella’, early – ‘Sante’, ‘Goda’, ‘VB Liepa’, medium – ‘Lady Rosetta’, ‘Red Lady’, ‘Courage’ and late – ‘VB Rasa’, ‘VB Aista’, ‘Saturna’, grown in Lithuania. Investigation of the influence of the maturity time of different potato varieties on the accumulation of the tested compounds suggested that very early potato varieties accumulated higher levels of biologically active compounds than the other varieties tested. The tubers of all varieties tested accumulated the highest concentrations of tyrosine, tryptophan, chlorogenic and caffeic acids were mostly accumulated by all tested varieties.

Quantification of biologically active compounds in the tubers of potato varieties of different maturity