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This research was aimed at exploring the antioxidative activity of new azole compounds and their influence on barley (Hordeum vulgare L.) growth in vitro and changes of flavonoid content. Azole derivative 5-{2-[(4-methylphenyl)amino]ethyl}-N-phenyl-1,3,4-thiadiazol-2-amine showed the best radical scavenging property. The highest growth regulating activity was demonstrated by 3-(4-methylphenyl)-1-phenyl-3-{2[5-(phenylamino)-1,3,4-thiadiazol-2-yl]ethyl}thiourea. The length of the barley shoots, which were exposed to this compound (0.1 mg l-1) increased by 42.7% in comparison with the control sample. Under its influence (0.4 mg l-1) barley roots were by 72.3% longer than the ones of the control sample. The highest antioxidative activity was determined for the barley seedlings, which were exposed to the same N′-thiocarbamoyl-1,3,4-thiadiazole (0.1 mg l-1).

Antioxidative activity of azoles and their influence on barley (Hordeum vulgare L.) seedlings and flavonoid accumulation