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Hamdani AD, Sulistiyowati E, Khusnuryani A. 2017. Soil improvement potential of weeds biomass applied as green manure in marginal land. Trop Drylands 1: 12-16. The presence of weeds that build up organic matter and chemical contents of soil may contribute to the soil maintenance of marginal land. This study aimed to assess the soil improvement potential Chromolaena odorata, Mikania micrantha, and Synedrella nodiflora biomass and the best form of their practical application in marginal land. Two forms of weed biomass application, fresh and dried chopped, were examined in a pot experiment containing soil from Patuk, Gunung Kidul, and spinach (Amaranthus tricolor L.) as the test crop. The pots were laid out in a randomized complete block design with six replications. Laboratory analysis showed that manurial properties between the weeds were statistically similar, with the average of organic C, N, P and K at 36.9, 3.3, 0.1 and 1.6%, respectively. Of the chemical contents considered, the content of all chemicals measured in experimental soil had increased, particularly the available K2O from 3.33 to the average of 46.33 ppm. The spinach growth and yield resulted from experimental soil were superior to that from untreated soil. However, there were no significant differences in the plants response between the types of weeds. Rather, better growth and yield of spinach resulted from soil treated with dried chopped weed biomass. Thus, weed biomass can be used to improve soil organic matter and therefore should be incorporated with the soil fertility maintenance.