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Harini M, Astirin OP. 2009. Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Nusantara Bioscience 1: 53-58. This study aims to determine treatment effect of VCO on blood cholesterol levels inhypercholesterolemic white rat (Rattus norvegicus L.). This study used 25 male rats of Wistar strain divided into five treatment groups,namely: control, simvastatin (1.3 mL/270 g BW), cholesterol (9:1 lard), VCO 1 (1 mL/270 g BW), and VCO 2 (1.3 mL/270 g BW). Treatment was given orally. Total cholesterol, LDL and HDL cholesterol levels were measured at day 1, day 14 and day 28. Cholesteroldata (total cholesterol, LDL and HDL) were analyzed by Ancova and followed by contrast test at significance level of 5%. The resultsshowed that treatment of VCO at different doses significantly affected the decrease in blood total cholesterol, blood LDL levels,increasing blood HDL in hipercholesterolemic white rat.