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Ayu VS, Nugraheni ER, Purwoko T. 2010. The effect of ethanol extracts concentration of mengkudu fruit (Morinda citrifolia) and storage time toward Euthynnus affinis meat quality. Biofarmasi 8: 58-65. High protein content in fish and the storage in a relatively short time will encourage the growth of bacteria. Therefore, the preservation techniques are required to expand the fish store. The use of formalin as a preservative substance, especially for fish, can damage to health, so it is necessary to find out the preservative substance as an alternative to substitute formalin. Mengkudu fruit (Morinda citrifolia L.) contains compounds that have an antibacterial activity, such as alkaloids and flavonoids. The purposes of this study were to determine the effect of ethanol extract concentration of mengkudu fruit and the storage time on the number of bacteria in Euthynnus affinis meat, and the concentration which have the same activity with formalin in inhibiting the number of bacteria in E. affinis meat. This research used a completely randomized design. The tested sample was Euthynnus affinis meat that soaked in ethanol extract of mengkudu fruit with concentrations between 10-100%. Every four hours, the TPC test, the water content, pH value and temperature of samples were measured. The results of observation were analyzed by ANOVA, further the results that significantly different were followed by a DMRT test with 95% confidence level. Based on the statistical analysis, the concentration of ethanol extract of mengkudu fruit and the storage time were significantly (p<0.05) in inhibiting the number of bacteria in Euthynnus affinis meat. The concentrations of extracts 80%, 90% and 100% had almost same activity to formalin in inhibiting the number of bacteria in Euthynnus affinis meat.