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The purposes of this research were to study the effect of amino acid DL-tryptophan at various concentrations on culture callus growth and production of alkaloid-reserpine of Rauvolfia serpentina and to determine optimum concentration of DL-tryptophan to yield maximum alkaloid reserpine of the callus. This research was consisted of three phases. First phase was to determine compatible sterilan for R. serpentina leaf explants. Second phase was to initiate/ to induce callus formation from the explants. Third phase was treatment phase to know the influence of DL-tryptophan addition on growth and alkaloid-reserpine production of the callus. Experimental design that used was Completely Randomized Design (CRD). The treatment was concentration of the amino acid DL- tryptophan with three levels concentration three restating for each level. The levels were 0 mg/L, 10 mg/L and 20 mg/L. Obtained data were analyzed quantitatively and qualitatively. Quantitative data were wet weight, dry weight, growth rate and alkaloid content of the callus at each level of concentration. Qualitative data have been measured were compatible sterilant test and callus morphology (color and texture). Quantitative data then have been analyzed by analysis of variance (ANOVA) and continued with DMRT test at level 95%. The result indicated that addition of DL- tryptophan precursor had an effect on the reduction of the callus growth of R. serpentina in media MS in vitro. Addition of DL-tryptophan precursor to produce of alkaloid-reserpine of the callus of R. serpentina in media MS in vitro and optimum concentration of DL-tryptophan precursor that must be added to yield of alkaloid-reserpine could not be determined yet, it caused by the alkaloidreserpine was not detected yet by the thin layer chromatography (TLC).

The effect of DL-tryptophan on callus growth and alkaloid-reserpin production of pule pandak [Rauvolfia serpentina (L.) Bentham ex Kurz] in vitro