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The studyaimsto characterize the Dickeya zeaeassociated with pineapple soft rot in East Lampung, Indonesia and to assess thebacterial host range.From the blister-like lesion-symptom, bacteria were isolated with the morphological characteristics: circular, convex, cream white milk-colored, with diameter coloniesranging from1-2 mm in diameter. Two strains (N-Unila 5 and N-Unila 10) were selected for further investigation including pathogenicity test on pineapple seedling, species identification by phenotypic characteristics and molecular techniques using sequence analysis of 16SrDNA, recA, and dnaXas well as host range test on 25 different plant species. The result ofthe pathogenicity test showedsimilarsymptomsto those observed in the field. Physiological and biochemical characterization revealed that the two isolates were Gram-negativebacteria, fermentative, lecithinase positive, non-fluorescenton King’s B medium,able to grow on YDC mediumat41oC, did not produce H2Sanddid notgrow in the presence of 5% NaCl. The isolates capable of using Myo-inositol, M-tartrate, mannitol, L-tartrate, lactose, glycerol, D-melibiose, D-arabinose, citrate, and cis-aconitic acid butdidnot utilize starch, S-ketoglucanate, L-ascorbic acid, inulin,folic acid, D-raffinose and tartrateas a sole carbon source.Phenotypic characteristics indicated that the strains were in the group of Dickeya spp. bv.3 (phenon 1). Sequence analysis of 16SrDNA, recA,and dnaXrevealed that the strains were placed in the same cluster with the referencestrain ofD. zeae.Host range assessment showed positive soft rot symptomsin Aloe vera, chinese cabbage, dragon-fruit, eggplant,lettuce, and welsh onion that have never been reported before

Characterization and host range assessment of Dickeya zeae associated with pineapple soft rot disease in East Lampung, Indonesia