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Marine ecosystem is rich with microorganisms such as bacteria and fungi either as freeliving or in association with macro-organisms. Marine invertebrates provide suitable habitats for fungi by supplying space, food, andother chemicals stuff that in some cases is a reciprocal relationship or called mutualism symbiotic. Some marine invertebrates haveinteresting activities that are useful for human life such as anticancer, antifungal, and antibacterial. Many reports indicated that thefungal growth and their production of bioactive compounds were highly affected by the media or nutrition. In order to understand theeffect of media on the number and bioactivity of the isolates, we collected the samples of marine invertebrates from two locations inMakassar. Invertebrate specimens were collected by hand during SCUBA diving at 3-10 m depths. The fungi were isolated by tappingmethod either on potato dextrose agar (PDA) or poor marine agar (PMA). The samples were collected from the Samalona water as muchas 16 specimens that provided 30 and 18 fungal isolates on PDA and PMA, respectively, while, from the Barrang Cadi water, a total 14specimens were collected to provide 12 and 3 isolates on PDA and PMA, respectively. All fungi from PMA inhibited the V. harveyi, V. vulnificus, and V. parahaemolyticus with weak, medium, and strong activities, while, the isolates from PDA were mostly not active against the Vibrios. Based on the molecular analyses, the active isolates were identified as Aspergillus flavus, A. oryzae, A. aculeatus, Talaromyces minioluteus, Hypocrea jecorina, Gliomastix murorum, Myrothecium inundatum, and Curvularia avinis. In conclusion, the isolates from PMA showed higher potential as source of antivibrio substances.

The effect of culture media on the number and bioactivity of marine invertebrates associated fungi