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Pujiastuti P, Masykuri M, Gunawan T, Sutarno. 2016. Bacterial spatial distribution in the sediments of Gajah Mungkur Reservoir, Central Java, Indonesia. Biodiversitas 17: 907-914. The study aims to obtain the spatial dynamic pattern of bacterial sediment in the Gajah Mungkur Reservoir, Wonogiri, Central Java, Indonesia. This study supplied with colony morphology characterization, gram staining and biochemical test on the sediment samples that were obtained from eight contaminated zones. Furthermore, the spatial dynamic map based on the distance function using contour interpolation technique is processed using ArcView GIS 10 software. The research result shows that the distribution pattern of bacterial diversity is dynamic enough, identified by grampositive bacteria: Bacillus sp., Bacillus cereus, and Staphylococcus sp.; and gram-negative bacteria: Klebsiella, Escherichia coli, Aeromonas hydrophila, Aeromonas sobria, Aeromonas caviae, Aeromonas schubertii, Plesiomonas shigelloides, Acinetobacter sp., and Pseudomonas (Comamonas) acidovorans. Gram-positive anaerobic bacteria show similar distribution pattern in all samples, including Clostridium sphenoides, and Clostridium paraputrificums.