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In this work we reported synthesis of long (from hundreds to thousands base pairs) linear and circular double stranded (ds) concatemers composed of tandem oligonucleotide repeats. The concatemers were produced following a method published [1] almost 40 years ago from 5’end phosphorylated half-complementary decameric or hexameric oligonucleotides (Fig. 1, left panel) as well from the oligonucleotides containing modified nucleic bases or mismatched ones. Using the above method we synthesized various uniform linear DNA polymers, including telomeric DNA, as well as long (kbp) repeating single stranded DNA

Synthesis of Long Double Stranded Concatemer Molecules by Self-Association of Short Single Stranded DNA Sequences